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3686s satb2 mouse  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc 3686s satb2 mouse
    3686s Satb2 Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p27/p27+Kip1+XP+Rabbit+mAb/pm41923199-255-68-66
    Average 96 stars, based on 711 article reviews
    3686s satb2 mouse - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    DNA Synthesis:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Staining:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Western Blot:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Protein-Protein interactions:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Fractionation:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Immunofluorescence:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Expressing:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Knockdown:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Stable Transfection:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Biomarker Discovery:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    CCK-8 Assay:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).

    Control:

    Article Title: BDH1 drives ovarian cancer progression by regulating cell cycling and stemness maintenance through Wnt/β-catenin signaling.
    Article Snippet: After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.After incubation with HRP-conjugated secondary antibodies (1:5000, Dako, cat. no. P0260), protein bands were visualized using ECL substrate (Pierce) and quantified with ImageJ.. The primary information of antibodies adopted in this work were listed below: BDH1 (1:1000; Proteintech, cat. no. 67448-1-Ig), pRb (1:1000; Cell Signaling Technology (CST), cat. no. #9308), CDK4 (1:1000; CST, cat. no. #12790), p21 (1:1000; CST, cat. no. #2947), p27 (1:1000; CST, cat. no. #3688), active-β-catenin (1:1000; CST, cat. no. #8814), Nanog (1:1000; CST, cat. no. #4903), SOX2 (1:1000; CST, cat. no. #23064), KLF4 (1:1000; CST, cat. no. #51221), and β-actin (1:2000; CST, cat. no. #8457).. Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were incubated overnight at 4°C with anti-active-β-catenin antibody (1:50; CST, cat no. #8814), followed by Alexa Fluor 488-conjugated secondary antibody (1:500; Invitrogen) for 1 h. Nuclei were counterstained with DAPI (Sigma).Immunofluorescence Cells grown on coverslips were fixed 48 h post-transfection with 4% PFA for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum..

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma.
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), panAkt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Bad (#10435-1-AP; Proteintech), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; Proteintech), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: Dual targeting of PI3Kδ and PPARα enhances antitumor activity via FoxO1 activation in follicular lymphoma
    Article Snippet: Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).Cytoplasmic and nuclear proteins were extracted using the NE-PER® Extraction Kit (Thermo Scientific).. The primary antibodies used were as follows: PI3K p110δ (A19742; ABclonal), pan-Akt (#4691; CST), phospho-Akt (Ser473, #4060; CST), PPARα (ab227074; Abcam), FoxO1 (#2880; CST), phospho-FoxO1 (Thr24, #9464; CST) and (Ser256, #9461; CST), FoxO3a (#12829; CST), FoxO4 (#9472; CST), Mcl-1 (#16225-1-AP; Proteintech), Bcl-2 (#12789-1-AP; Proteintech), Bim (A19702; ABclonal), Bax (#50599-2-Ig; Proteintech), Cleaved PARP(#5625; CST), P21(#2947; CST), p27 (#3686; CST), Cyclin E1 (#11935-1-AP; Proteintech), CDK2 (#10122-1-AP; Proteintech), phospho-CDK2 (#4539; CST), GLUT1(#73015; CST), PGK1(A12686; ABclonal), β-Tubulin (#10094-1-AP; Proteintech), Lamin B1 (#13435; CST), and β-Actin (#66009-1-Ig; Proteintech).. All full-length, uncropped western blot images are provided in the Supplemental Material.All full-length, uncropped western blot images are provided in the Supplemental Material.

    Article Title: UFD1 Recognition of Initiator and Proximal Ubiquitin Drives p97-Mediated Substrate Unfolding enhanced by FAF1, FAF2, and UBXD7
    Article Snippet: For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).For immunoblot assays, chemiluminescence signals were detected using a FUSION imaging system (Vilber-Lourmat).. The following antibodies were used: p21 (Cell Signaling Technologies, #2947, 1:1000), p27 (Cell Signaling Technologies, #3686, 1:1000), TXNIP (Cell Signaling Technologies, #14715, 1:1000), HIF-1α (Cell Signaling Technologies, #14179, 1:1000), FAF1 (Cell Signaling Technologies, #4932, 1:1000), UBXD7 (Thermo Fisher Scientific, #PA5-71280, 1:500), HA (Cell Signaling Technologies, #3724, 1:1000), and CDT1 (Cell Signaling Technologies, #8064, 1:1000).

    Article Title: Integrative Mechanistic Investigation of the Anticancer Effects of Panax notoginseng in Colorectal Cancer.
    Article Snippet: HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).HaltTM Protease and Phosphatase Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).. Antibodies against CDK4, Cyclin D1, CDK6, p27, p21, CDK2, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38, p-STAT3 (Tyr705), p-STAT3 (Ser727), STAT3, p-AKT, AKT, p-PI3K, PI3K, p-AMPKα, AMPKα, Cleaved PARP, Caspase9, Survivin, Caspase-8, Bcl-2, Mcl-1, HO-1, LC3A/B, and β-actin, as well as secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Imeglimin Exerts Anti‐Tumor Activity in Multiple Myeloma Through Affecting Energy Metabolism and Downregulating IL ‐16 Expression
    Article Snippet: L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).L(+)‐lactic acid was purchased from J&K Scientific (Beijing, China).. The antibodies for Ki‐67, Cyclin D3 antibody (CCND3), CDK4, p27, and p21 were purchased from Cell Signaling Technology (CST, MA, USA).



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